Mitomycin c prophage
WebNational Center for Biotechnology Information WebProphage Induction with mitomycin C (1ug/ml) gave me reduction in bacterial turbidity measured at OD 600 nm. A spot test of the lysate had given a noticeable clearance.
Mitomycin c prophage
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WebExposure to mitomycin C induces no DNA breakdown in these mutants (Boyce & Howard-Flanders, 1964). A recA uvr A double mutant was first constructed by Howard-Flander & Boycse (1966). The ultraviolet dose for 37 % survival of the double mutant is 0-2 erg/mm2, corresponding approximately to one dimer per bacterial genome (Howard-Flanders, 1968). WebThe results of the H. aquamarina phage (HAP-1) mitomycin C prophage in- duction experiments are shown in Fig. 1. In the mitomycin C-treated cultures, lytic activity was observed, ...
WebMitomycin C has also been used topically rather than intravenously in several areas. The first is cancers, particularly bladder cancers and intraperitoneal tumours. It is now well known that a single instillation of this agent within 6 hours of bladder tumor resection can prevent recurrence. WebIn the laboratory, the most common induction method is the DNA damaging chemical Mitomycin C. Although pH has been listed in the literature as an induction method, it is not widely used. Here, we detail a protocol for prophage induction by …
WebInduction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C Nature. 1959 Oct 3;184(Suppl 14):1079-80. doi: 10.1038/1841079b0. Authors N OTSUJI, M SEKIGUCHI, T IIJIMA, Y TAKAGI. PMID: 14429381 DOI: 10.1038/1841079b0 No abstract available. MeSH terms ... Web24 sep. 2024 · Genome analysis of representative strains of the 7 genetic lineages of Lactococcus lactis constituting the culture indicated the presence of prophages in …
WebTranscript levels of prophage-encoded genes in strain MGAS315 during culture with mitomycin C, hydrogen peroxide, and D562 epithelial cells relative to transcript levels under control conditions. Gene names are listed on the y axis.
Web24 aug. 2024 · When the culture reached an OD 600nm of 0.15–0.25, mitomycin C was added to 0.3 μg/ml, inverted several times for gentle mixing, and incubated at 37 °C in an anaerobic chamber for 15–20 h. flight ba43WebThe frequency of prophage carriage was tested in a collection of 108 clinical isolates of Streptococcus pneumoniae . A PCR-based assay was developed to allow classification of the prophage into the three groups recently identified according to genome chemicals flpWeb17 jun. 2024 · Previous data suggest that gene exchange may occur in Yersinia. Only scarce information exists about temperate phages of Y. enterocolitica, even though many prophage sequences are present in this species. We have examined 102 pathogenic Y. enterocolitica strains for the presence of inducible prophages by mitomycin C treatment. flight ba472WebVibrio vulnificus is a bacterium known to cause fatal necrotizing soft tissue infection in humans. Here, a remarkable therapeutic effect of hyperbaric oxygen (HBO) on V. vulnificus infection provoked by its injection into mouse footpads is described. HBO was shown to be bactericidal to this bacterium in vitro as well as in the infected tissue. The bactericidal … chemicals floorWeb1 dec. 1995 · A prophage was induced from cells of the pathogenic spirochaete Serpulina hyodysenteriae using mitomycin C. Five to seven hours after mitomycin C was added … chemicals firefighters are exposed toWebMitomycin C-treated bacteria resumed DNA synthesis also when infected with either T 3 or T 5 phage. 3. 3. Although mitomycin C did not affect the infectivity of free phage, the reproduction of phage was progressively impaired with increasing concentrations of the antibiotic. Phage-infected bacteria in contact with high levels of mitomycin C ... flight ba 49WebMost bacterial cells carry prophage genomes either integrated into the host DNA or present as repressed plasmids. Methods are described for the induction of prophages using … flight ba54