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WebThe 5′ capping Capping is an essential step for stability of nascently transcribed RNA and complete its life cycle successfully [ 35 ]. Otherwise, RNA is degraded by exonucleases. Capping is also necessary for further processing, splicing, localization and reading of RNAs. Process of capping begins alongwith transcription itself. Fundamental to the successful synthesis of longer oligos is the need to maintain as high a coupling efficiency as possible. While an average … See more The supports themselves are critical to maintaining a high coupling efficiency throughout the sequence. It is for that reason that we report … See more While it may not be readily apparent, it is critical to maintain a high capping efficiency when synthesizing long oligos. The reasons are two-fold: 1. There is the practical concern of … See more Purification difficulties can not be considered a side reaction per se, but we would be remiss not to mention that the purification of long … See more agra to bokaro distance
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